Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.
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ABSTRACT:
SUBMITTER: Mitzelfelt KA
PROVIDER: S-EPMC5355643 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection.
Mitzelfelt Katie A KA McDermott-Roe Chris C Grzybowski Michael N MN Marquez Maribel M Kuo Chieh-Ti CT Riedel Michael M Lai Shuping S Choi Melinda J MJ Kolander Kurt D KD Helbling Daniel D Dimmock David P DP Battle Michele A MA Jou Chuanchau J CJ Tristani-Firouzi Martin M Verbsky James W JW Benjamin Ivor J IJ Geurts Aron M AM
Stem cell reports 20170224 3
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease ...[more]
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