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Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species.


ABSTRACT: We evaluated a multiplex real-time PCR and melting curve analysis assay (Real-Q NTM-ID kit; Biosewoom, Seoul, South Korea) for the identification of eight common nontuberculous mycobacterial species, using 30 type strains and 230 consecutive clinical isolates. The concordance rate of this assay with multigene sequence-based typing was 97.0% (223/230 isolates).

SUBMITTER: Huh HJ 

PROVIDER: S-EPMC4313269 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species.

Huh Hee Jae HJ   Park Kyung Sun KS   Jang Mi-Ae MA   Kim Ji-Youn JY   Kwon Hyeon Jeong HJ   Ki Chang-Seok CS   Lee Nam Yong NY  

Journal of clinical microbiology 20140827 11


We evaluated a multiplex real-time PCR and melting curve analysis assay (Real-Q NTM-ID kit; Biosewoom, Seoul, South Korea) for the identification of eight common nontuberculous mycobacterial species, using 30 type strains and 230 consecutive clinical isolates. The concordance rate of this assay with multigene sequence-based typing was 97.0% (223/230 isolates). ...[more]

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