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Quantitative residue-specific protein backbone torsion angle dynamics from concerted measurement of 3J couplings.


ABSTRACT: Three-bond (3)J(C'C') and (3)J(HNH?) couplings in peptides and proteins are functions of the intervening backbone torsion angle ?. In well-ordered regions, (3)J(HNH?) is tightly correlated with (3)J(C'C'), but the presence of large ? angle fluctuations differentially affects the two types of couplings. Assuming the ? angles follow a Gaussian distribution, the width of this distribution can be extracted from (3)J(C'C') and (3)J(HNH?), as demonstrated for the folded proteins ubiquitin and GB3. In intrinsically disordered proteins, slow transverse relaxation permits measurement of (3)J(C'C') and (3)J(HNH) couplings at very high precision, and impact of factors other than the intervening torsion angle on (3)J will be minimal, making these couplings exceptionally valuable structural reporters. Analysis of ?-synuclein yields rather homogeneous widths of 69 ± 6° for the ? angle distributions and (3)J(C'C') values that agree well with those of a recent maximum entropy analysis of chemical shifts, J couplings, and (1)H-(1)H NOEs. Data are consistent with a modest (?30%) population of the polyproline II region.

SUBMITTER: Lee JH 

PROVIDER: S-EPMC4318723 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Quantitative residue-specific protein backbone torsion angle dynamics from concerted measurement of 3J couplings.

Lee Jung Ho JH   Li Fang F   Grishaev Alexander A   Bax Ad A  

Journal of the American Chemical Society 20150123 4


Three-bond (3)J(C'C') and (3)J(HNHα) couplings in peptides and proteins are functions of the intervening backbone torsion angle ϕ. In well-ordered regions, (3)J(HNHα) is tightly correlated with (3)J(C'C'), but the presence of large ϕ angle fluctuations differentially affects the two types of couplings. Assuming the ϕ angles follow a Gaussian distribution, the width of this distribution can be extracted from (3)J(C'C') and (3)J(HNHα), as demonstrated for the folded proteins ubiquitin and GB3. In  ...[more]

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