Ontology highlight
ABSTRACT:
SUBMITTER: Lee JH
PROVIDER: S-EPMC4318723 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Lee Jung Ho JH Li Fang F Grishaev Alexander A Bax Ad A
Journal of the American Chemical Society 20150123 4
Three-bond (3)J(C'C') and (3)J(HNHα) couplings in peptides and proteins are functions of the intervening backbone torsion angle ϕ. In well-ordered regions, (3)J(HNHα) is tightly correlated with (3)J(C'C'), but the presence of large ϕ angle fluctuations differentially affects the two types of couplings. Assuming the ϕ angles follow a Gaussian distribution, the width of this distribution can be extracted from (3)J(C'C') and (3)J(HNHα), as demonstrated for the folded proteins ubiquitin and GB3. In ...[more]