Ontology highlight
ABSTRACT:
SUBMITTER: Goswami GK
PROVIDER: S-EPMC4320173 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Goswami Girish K GK Krishnamohan Medichtrla M Nain Vikrant V Aggarwal Chetana C Ramesh Bandarupalli B
SpringerPlus 20140110
Xylanase gene isolated from Bacillus brevis was expressed in E. coli BL21. Sequencing of the gene (Gen Bank accession number: HQ179986) showed that it belongs to family 11 xylanases. The recombinant xylanase was predominantly secreted to culture medium and showed mesophilic nature (optimum activity at 55°C and pH 7.0). The cell free culture medium exhibited 30 IU/ml xylanse activity. The enzyme did not show any cellulose activity and was active under wide range of temperature (40°C to 80°C) and ...[more]