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Cloning and heterologous expression of cellulose free thermostable xylanase from Bacillus brevis.


ABSTRACT: Xylanase gene isolated from Bacillus brevis was expressed in E. coli BL21. Sequencing of the gene (Gen Bank accession number: HQ179986) showed that it belongs to family 11 xylanases. The recombinant xylanase was predominantly secreted to culture medium and showed mesophilic nature (optimum activity at 55°C and pH 7.0). The cell free culture medium exhibited 30 IU/ml xylanse activity. The enzyme did not show any cellulose activity and was active under wide range of temperature (40°C to 80°C) and pH (4 to 9). The enzyme showed considerable thermo stability and regained over 90% of activity, when returned to 55°C after boiling for 5 min. These physiochemical properties of B. brevis xylanse show high potential of its applications in paper and pulp industry.

SUBMITTER: Goswami GK 

PROVIDER: S-EPMC4320173 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Cloning and heterologous expression of cellulose free thermostable xylanase from Bacillus brevis.

Goswami Girish K GK   Krishnamohan Medichtrla M   Nain Vikrant V   Aggarwal Chetana C   Ramesh Bandarupalli B  

SpringerPlus 20140110


Xylanase gene isolated from Bacillus brevis was expressed in E. coli BL21. Sequencing of the gene (Gen Bank accession number: HQ179986) showed that it belongs to family 11 xylanases. The recombinant xylanase was predominantly secreted to culture medium and showed mesophilic nature (optimum activity at 55°C and pH 7.0). The cell free culture medium exhibited 30 IU/ml xylanse activity. The enzyme did not show any cellulose activity and was active under wide range of temperature (40°C to 80°C) and  ...[more]

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