Ontology highlight
ABSTRACT:
SUBMITTER: Cho WK
PROVIDER: S-EPMC4320747 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20140522 26
Sliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA. We followed events during loading of an E. coli β clamp in real time by using single-molecule FRET (smFRET). Three successive FRET states were retained for 0.3 s, 0.7 s, and 9 min: Hydrolysis of the first ATP molecule by the γ clamp loader resulted in closure of the cl ...[more]