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Synthetic polymers as substrates for a DNA-sliding clamp protein.

ABSTRACT: The clamp protein (gp45) of the DNA polymerase III of the bacteriophage T4 is known to bind to DNA and stay attached to it in order to facilitate the process of DNA copying by the polymerase. As part of a project aimed at developing new biomimetic data-encoding systems we have investigated the binding of gp45 to synthetic polymers, that is, rigid, helical polyisocyanopeptides. Molecular modelling studies suggest that the clamp protein may interact with the latter polymers. Experiments aimed at verifying these interactions are presented and discussed.

SUBMITTER: van Dongen SFM

PROVIDER: S-EPMC6001473 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Synthetic polymers as substrates for a DNA-sliding clamp protein.

van Dongen S F M SFM Clerx J J van den Boomen O I OI Pervaiz M M Trakselis M A MA Ritschel T T Schoonen L L Schoenmakers D C DC Nolte R J M RJM

Biopolymers 20180426 5

The clamp protein (gp45) of the DNA polymerase III of the bacteriophage T4 is known to bind to DNA and stay attached to it in order to facilitate the process of DNA copying by the polymerase. As part of a project aimed at developing new biomimetic data-encoding systems we have investigated the binding of gp45 to synthetic polymers, that is, rigid, helical polyisocyanopeptides. Molecular modelling studies suggest that the clamp protein may interact with the latter polymers. Experiments aimed at v ...[more]

PMID: 29700825

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