Dataset Information

Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA.

ABSTRACT: AIM:To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs). METHODS:Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19 x 10(12) copies of linear HBV DNA/1 x 10(7) PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System. Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group. RESULTS:HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive > or =2.1) respectively. HBeAg was negative (<2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected group. Dot blotting revealed that HBV DNA was strongly positive in the transfected group and negative in the control group. Southern blot analysis of intracellular total DNA indicated that there were relaxed circular (rc DNA), covalently closed circular (ccc DNA), and single-stranded (ss DNA) HBV DNA replicative intermediates in the transfected group, there was no integrated HBV DNA in the cellular genome. These parameters were negative in control group. CONCLUSION:Expression and replication of HBV genes can occur in hepatocytes from non-mammalian species. HBV replication has no critical species-specificity, and yet hepatic-specific regulating factors in hepatocytes may be essential for viral replication.

SUBMITTER: Yao YQ

PROVIDER: S-EPMC4321921 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature

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Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA.

Yao Yun-Qing YQ Zhang Ding-Feng DF Tang Ni N Huang Ai-Long AL Zou Xiao-Yi XY Xiao Jiang-Feng JF Luo Yun Y Zhang Da-Zhi DZ Wang Bo B Zhou Wei-Ping WP Ren Hong H Liu Qi Q Guo Shu-Hua SH

World journal of gastroenterology 20050801 32

<h4>Aim</h4>To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).<h4>Methods</h4>Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19 x 10(12) copies of linear HBV DNA/1 x 10(7) PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System. Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs ...[more]

PMID: 16124057

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Dataset Information

Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA.

Publications

Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA.

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