Ontology highlight
ABSTRACT:
SUBMITTER: Mankowski WC
PROVIDER: S-EPMC4324458 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Mankowski Walter C WC Winter Mark R MR Wait Eric E Lodder Mels M Schumacher Ton T Naik Shalin H SH Cohen Andrew R AR
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference 20140101
Image sequences of live proliferating cells often contain visual ambiguities that are difficult even for human domain experts to resolve. Here we present a new approach to analyzing image sequences that capture the development of clones of hematopoietic stem cells (HSCs) from live cell time lapse microscopy. The HSCs cannot survive long term imaging unless they are cultured together with a secondary cell type, OP9 stromal cells. The HSCs frequently disappear under the OP9 cell layer, making segm ...[more]