Ontology highlight
ABSTRACT:
SUBMITTER: Lu R
PROVIDER: S-EPMC3196379 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Lu Rong R Neff Norma F NF Quake Stephen R SR Weissman Irving L IL
Nature biotechnology 20111002 10
Disentangling cellular heterogeneity is a challenge in many fields, particularly in the stem cell and cancer biology fields. Here we demonstrate how to combine viral genetic barcoding with high-throughput sequencing to track single cells in a heterogeneous population. We use this technique to track the in vivo differentiation of unitary hematopoietic stem cells (HSCs). The results are consistent with single-cell transplantation studies but require two orders of magnitude fewer mice. In addition ...[more]