Unknown

Dataset Information

0

Constitutive mitochondrial DNA copy number in peripheral blood of melanoma families with and without CDKN2A mutations.


ABSTRACT: Quantitative changes in mitochondrial DNA (mtDNA) have been associated with the risk of a number of human cancers; however, the relationship between constitutive mtDNA copy number in blood and the risk of familial cutaneous malignant melanoma (CMM) has not been reported. We measured mtDNA copy number using quantitative PCR in blood-derived DNA from 136 CMM cases and 302 controls in 53 melanoma-prone families (23 segregating CDKN2A germline mutations). MtDNA copy number did not vary by age, sex, pigmentation characteristics, or CMM status. However, germline CDKN2A mutation carriers had significantly higher mean mtDNA copy number compared to non-carriers, particularly among CMM cases (geometric mean mtDNA copy number of 144 and 111 for carrier versus non-carrier, respectively; P= 0.02). When adjusting for age, sex, and familial correlation, having increasing mtDNA copy number was significantly associated with CDKN2A mutation status among CMM cases (OR=1.47, Ptrend=0.024). In particular, individuals with specific CDKN2A mutations with the potential to inactivate or reduce the level of the p16-INK4 reactive oxygen species (ROS) protective function had significantly increased mtDNA copy number levels (P=0.035). Future research in prospective studies is required to validate these findings and to further investigate mtDNA copy number in both blood and melanoma tissues in relation to CMM risk and CDKN2A mutation status.

SUBMITTER: Hyland PL 

PROVIDER: S-EPMC4326067 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Constitutive mitochondrial DNA copy number in peripheral blood of melanoma families with and without <i>CDKN2A</i> mutations.

Hyland Paula L PL   Pfeiffer Ruth M RM   Rotunno Melissa M   Hofmann Jonathan N JN   Liu Chin-San CS   Cheng Wen-Ling WL   Yuenger Jeff J   Lan Qing Q   Tucker Margaret A MA   Goldstein Alisa M AM   Yang Xiaohong R XR  

Journal of carcinogenesis & mutagenesis 20140601 Suppl 4


Quantitative changes in mitochondrial DNA (mtDNA) have been associated with the risk of a number of human cancers; however, the relationship between constitutive mtDNA copy number in blood and the risk of familial cutaneous malignant melanoma (CMM) has not been reported. We measured mtDNA copy number using quantitative PCR in blood-derived DNA from 136 CMM cases and 302 controls in 53 melanoma-prone families (23 segregating <i>CDKN2A</i> germline mutations). MtDNA copy number did not vary by age  ...[more]

Similar Datasets

| S-EPMC7297203 | biostudies-literature
| S-EPMC4482392 | biostudies-other
| S-EPMC2767393 | biostudies-literature
| S-EPMC3747185 | biostudies-literature
| S-EPMC6916246 | biostudies-literature
| S-EPMC3274649 | biostudies-literature
| S-EPMC3233727 | biostudies-literature
| S-EPMC1226062 | biostudies-literature
| S-EPMC2080476 | biostudies-literature
| S-EPMC3244023 | biostudies-literature