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Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe.

ABSTRACT: Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.

SUBMITTER: Kim D

PROVIDER: S-EPMC4329546 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe.

Kim Dabin D Kim Gyoungmi G Nam Sang-Jip SJ Yin Jun J Yoon Juyoung J

Scientific reports 20150216

Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was use ...[more]

PMID: 25684681

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