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Identification of the 5' end of Rous sarcoma virus RNA.

ABSTRACT: The 5'-terminal triphosphate of the 35S RNA isolated from Rous sarcoma virus is blocked by 7-methylguanosine in 5' linkage with the penultimate nucleoside which is methylated in the 2'-O-ribose position, a type of endgroup found in all animal mRNAs investigated during the past year. The specific nuclease-resistant terminal fragment of RSV RNA has the structure 7mG5'ppp5'GmpCp-. This finding supports the belief that RNA of Rous sarcoma virus represents a (+) strand messenger which may be directly translated upon infection.

SUBMITTER: Keith J

PROVIDER: S-EPMC432989 | biostudies-literature | 1975 Sep

REPOSITORIES: biostudies-literature

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Identification of the 5' end of Rous sarcoma virus RNA.

Keith J J Fraenkel-Conrat H H

Proceedings of the National Academy of Sciences of the United States of America 19750901 9

The 5'-terminal triphosphate of the 35S RNA isolated from Rous sarcoma virus is blocked by 7-methylguanosine in 5' linkage with the penultimate nucleoside which is methylated in the 2'-O-ribose position, a type of endgroup found in all animal mRNAs investigated during the past year. The specific nuclease-resistant terminal fragment of RSV RNA has the structure 7mG5'ppp5'GmpCp-. This finding supports the belief that RNA of Rous sarcoma virus represents a (+) strand messenger which may be directly ...[more]

PMID: 171660

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