High-throughput detection of DNA double-strand breaks using image cytometry.
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ABSTRACT:
SUBMITTER: Fowler TL
PROVIDER: S-EPMC4331074 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Publications
High-throughput detection of DNA double-strand breaks using image cytometry.
Fowler Tyler L TL Bailey Alison M AM Bednarz Bryan P BP Kimple Randall J RJ
BioTechniques 20150101 1
Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method ...[more]
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