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Detection of DNA double-strand breaks in T-47D cells using DSBCapture-seq

ABSTRACT: Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3B, A3B) is a key molecular driver inducing mutations in multiple human cancer. A3B belongs to the APOBEC3 enzyme family, which consists seven closely related DNA deaminases that catalyse cytosine-to-uracil (C>U) editing of single-stranded DNA (ssDNA). Here, we used DSBCapture-seq to study the formation of DNA double-strand breaks (DSB) by estrogen receptor activation upon 17-estradiol (E2) induction. The effect of A3B-depletion on DSB formation was investigated using RNA interference.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193230 | GEO | 2025/01/06

REPOSITORIES: GEO

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ChIP-seq of TDRD3, CARM1 and H3R17me2a in T-47D cells

Project description:Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3B, A3B) is a key molecular driver inducing mutations in multiple human cancer. A3B belongs to the APOBEC3 enzyme family, which consists seven closely related DNA deaminases that catalyse cytosine-to-uracil (C>U) editing of single-stranded DNA (ssDNA). By using mass-spec proteomics, we identified TDRD3, a ‘reader’ of asymmetrically methylated arginine marks, as the binding partner of A3B.

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