Dataset Information

Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

ABSTRACT: Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening.

SUBMITTER: Dautt-Castro M

PROVIDER: S-EPMC4332321 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

Dautt-Castro Mitzuko M Ochoa-Leyva Adrian A Contreras-Vergara Carmen A CA Pacheco-Sanchez Magda A MA Casas-Flores Sergio S Sanchez-Flores Alejandro A Kuhn David N DN Islas-Osuna Maria A MA

Frontiers in plant science 20150218

Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding ...[more]

PMID: 25741352

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Project description:Climate change is becoming a global problem because of its harmful effects on crop productivity. In this regard, it is crucial to carry out studies to determine crops' response to heatwave stress. Response molecular mechanisms during the development and ripening of mango fruit (Mangifera indica L. cv. Chaunsa White) under extreme heatwaves were studied. Mango flowers were tagged and fruits 18, 34, 62, 79, 92 days after flowering (DAF) as well as fruits on 10 and 15 days of postharvest shelf life were studied through RNA-Seq and metabolome of the fruit mesocarp. The environmental temperature was recorded during the experiment. Roughly, 2 000 000 clean reads were generated and assembled into 12 876 redundant transcripts and 2674 non-redundant transcripts. The expression of genes playing a role in oxidative stress, circadian rhythm, senescence, glycolysis, secondary metabolite biosynthesis, flavonoid biosynthesis and monoterpenoid biosynthesis was quantified as well as reactive oxygen species. Higher expressions of six abiotic stress genes and a senescent associated gene was found at 79 DAF (recorded temperature 44 °C). Higher expressions of nucleoredoxin and glutathione S-transferase 1 family protein were also recorded. Activation of the GABA-shunt pathway was detected by the glutamate decarboxylase transcript expression at 79 DAF. Larger energy demands at the beginning of fruit ripening were indicated by an increase in fructose-bisphosphate aldolase gene expression. Finally, the radical-scavenging effect of mango fruit inflorescence and fruit pulp extracts showed decline upon heatwave exposure. We recorded a broad genetic response of mango fruit suggesting the activation of several metabolic pathways which indicated the occurrence of genetic and metabolic crosstalks in response to intense heatwaves. Collectively, this study presents experimental evidence to help in the elucidation of the molecular mechanism of crops response to heat stress which in turn will help in the designing of protocols to increase crop productivity in the face of climate change.

Dataset Information

Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

Publications

Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

Similar Datasets

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