Unknown

Dataset Information

0

Class-switched memory B cells remodel BCRs within secondary germinal centers.


ABSTRACT: Effective vaccines induce high-affinity memory B cells and durable antibody responses through accelerated mechanisms of natural selection. Secondary changes in antibody repertoires after vaccine boosts suggest progressive rediversification of B cell receptors (BCRs), but the underlying mechanisms remain unresolved. Here, the integrated specificity and function of individual memory B cell progeny revealed ongoing evolution of polyclonal antibody specificities through germinal center (GC)-specific transcriptional activity. At the clonal and subclonal levels, single-cell expression of the genes encoding the costimulatory molecule CD83 and the DNA polymerase Pol? segregated the secondary GC transcriptional program into four stages that regulated divergent mechanisms of memory BCR evolution. Our studies demonstrate that vaccine boosts reactivate a cyclic program of GC function in class-switched memory B cells to remodel existing antibody specificities and enhance durable immunological protection.

SUBMITTER: McHeyzer-Williams LJ 

PROVIDER: S-EPMC4333102 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Class-switched memory B cells remodel BCRs within secondary germinal centers.

McHeyzer-Williams Louise J LJ   Milpied Pierre J PJ   Okitsu Shinji L SL   McHeyzer-Williams Michael G MG  

Nature immunology 20150202 3


Effective vaccines induce high-affinity memory B cells and durable antibody responses through accelerated mechanisms of natural selection. Secondary changes in antibody repertoires after vaccine boosts suggest progressive rediversification of B cell receptors (BCRs), but the underlying mechanisms remain unresolved. Here, the integrated specificity and function of individual memory B cell progeny revealed ongoing evolution of polyclonal antibody specificities through germinal center (GC)-specific  ...[more]

Similar Datasets

| S-EPMC7502816 | biostudies-literature
2020-02-21 | GSE140795 | GEO
| S-EPMC2118493 | biostudies-literature
| S-EPMC6914312 | biostudies-literature
| S-EPMC2901464 | biostudies-literature
| PRJNA591005 | ENA
2015-07-07 | E-MTAB-3637 | biostudies-arrayexpress
| S-EPMC9613510 | biostudies-literature
| S-EPMC18959 | biostudies-literature
| S-EPMC8516727 | biostudies-literature