Ontology highlight
ABSTRACT:
SUBMITTER: Myllykangas S
PROVIDER: S-EPMC4336783 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Nature biotechnology 20111023 11
We describe an approach for targeted genome resequencing, called oligonucleotide-selective sequencing (OS-Seq), in which we modify the immobilized lawn of oligonucleotide primers of a next-generation DNA sequencer to function as both a capture and sequencing substrate. We apply OS-Seq to resequence the exons of either 10 or 344 cancer genes from human DNA samples. In our assessment of capture performance, >87% of the captured sequence originated from the intended target region with sequencing co ...[more]