Photoreceptor cells display a daily rhythm in the orphan receptor Esrr?.

ABSTRACT: PURPOSE: Nuclear orphan receptors are critical for the development and long-term survival of photoreceptor cells. In the present study, the expression of the nuclear orphan receptor Esrr?--a transcriptional regulator of energy metabolism that protects rod photoreceptors from dystrophy--was tested under daily regulation in the retina and photoreceptor cells. METHODS: The daily transcript and protein amount profiles were recorded in preparations of the whole retina and microdissected photoreceptor cells using quantitative PCR (qPCR) and western blot analysis. RESULTS: Esrr? displayed a daily rhythm with elevated values at night in the whole retina and enriched photoreceptor cells. Daily regulation of Esrr? mRNA depended on light input but not on melatonin, and evoked a corresponding rhythm in the Esrr? protein. CONCLUSIONS: The data presented in this study indicate that daily regulation of Esrr? in photoreceptor cells may contribute to their adaptation to 24-h changes in metabolic demands.

SUBMITTER: Kunst S 

PROVIDER: S-EPMC4337357 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature