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Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae.

ABSTRACT: We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells and, iv) the periodicity of both glycolytic oscillations and dipolar relaxation are equally affected by D2O in a dose-dependent manner. These results offer a new insight into the coupling of an emergent intensive physicochemical property of the cell, i.e. cell-wide water dipolar relaxation, and a central metabolite (ATP) produced by a robustly oscillating metabolic process.

SUBMITTER: Thoke HS

PROVIDER: S-EPMC4338026 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Tight coupling of metabolic oscillations and intracellular water dynamics in Saccharomyces cerevisiae.

Thoke Henrik Seir HS Tobiesen Asger A Brewer Jonathan J Hansen Per Lyngs PL Stock Roberto P RP Olsen Lars F LF Bagatolli Luis A LA

PloS one 20150223 2

We detected very strong coupling between the oscillating concentration of ATP and the dynamics of intracellular water during glycolysis in Saccharomyces cerevisiae. Our results indicate that: i) dipolar relaxation of intracellular water is heterogeneous within the cell and different from dilute conditions, ii) water dipolar relaxation oscillates with glycolysis and in phase with ATP concentration, iii) this phenomenon is scale-invariant from the subcellular to the ensemble of synchronized cells ...[more]

PMID: 25705902

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