A simple real-time assay for in vitro translation.
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ABSTRACT:
SUBMITTER: Capece MC
PROVIDER: S-EPMC4338355 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Publications
A simple real-time assay for in vitro translation.
Capece Mark C MC Kornberg Guy L GL Petrov Alexey A Puglisi Joseph D JD
RNA (New York, N.Y.) 20141218 2
A high-throughput assay for real-time measurement of translation rates in cell-free protein synthesis (SNAP assay) is described. The SNAP assay enables quantitative, real-time measurement of overall translation rates in vitro via the synthesis of O(6)-alkylguanine DNA O(6)-alkyltransferase (SNAP). SNAP production is continuously detected by fluorescence produced by the reaction of SNAP with a range of quenched fluorogenic substrates. The capabilities of the assay are exemplified by measurements ...[more]
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