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Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.


ABSTRACT: Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC434119 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

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Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.

Wang Zhi Z   Ren Lili L   Zhao Xingang X   Hung Tao T   Meng Anming A   Wang Jianwei J   Chen Ye-Guang YG  

Journal of virology 20040701 14


Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV ge  ...[more]

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