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Highly stable and sensitive fluorescent probes (LysoProbes) for lysosomal labeling and tracking.

ABSTRACT: We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis.

SUBMITTER: Yapici NB

PROVIDER: S-EPMC4341211 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Highly stable and sensitive fluorescent probes (LysoProbes) for lysosomal labeling and tracking.

Yapici Nazmiye B NB Bi Yue Y Li Pengfei P Chen Xin X Yan Xin X Mandalapu Srinivas Rao SR Faucett Megan M Jockusch Steffen S Ju Jingfang J Gibson K Michael KM Pavan William J WJ Bi Lanrong L

Scientific reports 20150226

We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide ...[more]

PMID: 25715948

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