Unknown

Dataset Information

0

Real-time PCR for dihydrofolate reductase gene single-nucleotide polymorphisms in Plasmodium vivax isolates.

ABSTRACT: Mutations in the dhfr gene of Plasmodium vivax (pvdhfr) are associated with resistance to the antifolate antimalarial drugs. Polymorphisms in the pvdhfr gene were assessed by hybridization probe technology on the LightCycler instrument with 134 P. vivax-infected blood samples from Turkey (n = 24), Azerbaijan (n = 39), Thailand (n = 16), Indonesia (n = 53), and travelers (n = 19). Double mutations (S58R and S117N) or quadruple mutations (F57L/I, S58R, T61M, and S117N) in the pvdhfr genes were found in all Thai samples (100%). pvdhfr mutant-type alleles were significantly more common in samples from travelers (42%) than in those from patients from Indonesia (5%). Surprisingly, the pvdhfr single-mutation allele (S117N) was identified at a high frequency in parasites from Turkey and Azerbaijan (71 and 36%, respectively), where sulfadoxine-pyrimethamine is not recommended for the treatment of P. vivax malaria by the World Health Organization and the Malaria National Programs.

SUBMITTER: Brega S 

PROVIDER: S-EPMC434159 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Real-time PCR for dihydrofolate reductase gene single-nucleotide polymorphisms in Plasmodium vivax isolates.

Brega Sara S   de Monbrison Frédérique F   Severini Carlo C   Udomsangpetch Rachanee R   Sutanto Inge I   Ruckert Paul P   Peyron François F   Picot Stéphane S  

Antimicrobial agents and chemotherapy 20040701 7

Mutations in the dhfr gene of Plasmodium vivax (pvdhfr) are associated with resistance to the antifolate antimalarial drugs. Polymorphisms in the pvdhfr gene were assessed by hybridization probe technology on the LightCycler instrument with 134 P. vivax-infected blood samples from Turkey (n = 24), Azerbaijan (n = 39), Thailand (n = 16), Indonesia (n = 53), and travelers (n = 19). Double mutations (S58R and S117N) or quadruple mutations (F57L/I, S58R, T61M, and S117N) in the pvdhfr genes were fou  ...[more]

Similar Datasets

Unknown Analysis of the dihydrofolate reductase-thymidylate synthase gene sequences in Plasmodium vivax field isolates that failed chloroquine treatment.
| S-EPMC2999615 | biostudies-literature
Unknown Pyrimethamine and WR99210 exert opposing selection on dihydrofolate reductase from Plasmodium vivax.
| S-EPMC130599 | biostudies-literature
Unknown Mutations in the antifolate-resistance-associated genes dihydrofolate reductase and dihydropteroate synthase in Plasmodium vivax isolates from malaria-endemic countries.
| S-EPMC2929037 | biostudies-literature
Unknown Functional analysis of Plasmodium vivax dihydrofolate reductase-thymidylate synthase genes through stable transformation of Plasmodium falciparum.
| S-EPMC3392216 | biostudies-literature
Unknown An unlabelled probe-based real time PCR and modified semi-nested PCR as molecular tools for analysis of chloroquine resistant Plasmodium vivax isolates from Afghanistan.
| S-EPMC7362495 | biostudies-literature
Unknown Single-nucleotide polymorphisms and genome diversity in Plasmodium vivax.
| S-EPMC166258 | biostudies-literature
Unknown PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates.
| S-EPMC5450958 | biostudies-literature
Unknown Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance.
| S-EPMC1201571 | biostudies-literature
Unknown Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis.
| S-EPMC356834 | biostudies-literature
Unknown Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades.
| S-EPMC2224295 | biostudies-literature