Sequence tag-based analysis of microbial population dynamics.
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ABSTRACT:
SUBMITTER: Abel S
PROVIDER: S-EPMC4344388 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Sequence tag-based analysis of microbial population dynamics.
Abel Sören S Abel zur Wiesch Pia P Chang Hsiao-Han HH Davis Brigid M BM Lipsitch Marc M Waldor Matthew K MK
Nature methods 20150119 3
We describe sequence tag-based analysis of microbial populations (STAMP) for characterization of pathogen population dynamics during infection. STAMP analyzes the frequency changes of genetically 'barcoded' organisms to quantify population bottlenecks and infer the founding population size. Analyses of intraintestinal Vibrio cholerae revealed infection-stage and region-specific host barriers to infection and showed unexpected V. cholerae migration counter to intestinal flow. STAMP provides a rob ...[more]
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