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ABSTRACT:
SUBMITTER: Grimm JB
PROVIDER: S-EPMC4344395 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Grimm Jonathan B JB English Brian P BP Chen Jiji J Slaughter Joel P JP Zhang Zhengjian Z Revyakin Andrey A Patel Ronak R Macklin John J JJ Normanno Davide D Singer Robert H RH Lionnet Timothée T Lavis Luke D LD
Nature methods 20150119 3
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside living cells, resulting in brighter reporters than fluorescent proteins. Intracellular labeling using these techniques requires cell-permeable fluorescent ligands, however, limiting utility to a small number of classic fluorophores. Here we describe a simple structural modification that improves the brightnes ...[more]