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Switchable Fluorophores for Single-Molecule Localization Microscopy.

ABSTRACT: The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.

SUBMITTER: Li H

PROVIDER: S-EPMC6476321 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Switchable Fluorophores for Single-Molecule Localization Microscopy.

Li Honglin H Vaughan Joshua C JC

Chemical reviews 20180917 18

The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review ...[more]

PMID: 30221931

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