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Characterization of bovine interferon ?1: expression in yeast Pichia pastoris, biological activities, and physicochemical characteristics.

ABSTRACT: A bovine interferon ? (BoIFN?) gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-?1 according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino-acid signal peptide and a 166-amino-acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-?1 gene were analyzed. A recombinant mature BoIFN-?1 (rBoIFN-?1) was expressed in the yeast Pichia pastoris. Physicochemical characteristics and antiviral activity were determined in vitro. Recombinant BoIFN-?1 was found to be highly sensitive to trypsin and stable at pH 2.0 or 65°C. It also exhibited antiviral activity, which was neutralized by a rabbit anti-rBoIFN? polyclonal antibody. This study revealed that rBoIFN-?1 has the typical characteristics of IFN? and can be used for both research and industrial application.

SUBMITTER: Shao J 

PROVIDER: S-EPMC4350256 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Characterization of bovine interferon α1: expression in yeast Pichia pastoris, biological activities, and physicochemical characteristics.

Shao Jianwei J   Cao Chong C   Bao Jun J   Liu Hongtao H   Peng Tongquan T   Gao Mingchun M   Wang Junwei J  

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 20141024 3

A bovine interferon α (BoIFNα) gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-α1 according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino-acid signal peptide and a 166-amino-acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-α1 gene were analyzed. A recombinant mature BoIFN-α1 (rBoIFN-α1) was expressed in the yeast  ...[more]

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