Ontology highlight
ABSTRACT:
SUBMITTER: Ramachandran S
PROVIDER: S-EPMC4352886 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Ramachandran Srinivas S Zentner Gabriel E GE Henikoff Steven S
Genome research 20141209 3
Nucleosomes in active chromatin are dynamic, but whether they have distinct structural conformations is unknown. To identify nucleosomes with alternative structures genome-wide, we used H4S47C-anchored cleavage mapping, which revealed that 5% of budding yeast (Saccharomyces cerevisiae) nucleosome positions have asymmetric histone-DNA interactions. These asymmetric interactions are enriched at nucleosome positions that flank promoters. Micrococcal nuclease (MNase) sequence-based profiles of asymm ...[more]