Ontology highlight
ABSTRACT:
SUBMITTER: Twahir UT
PROVIDER: S-EPMC4355160 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Free radical biology & medicine 20141216
This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin-trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid, the hydroperoxyl radical, is expected as an intermediate in the decarboxylation and oxidation reactions of the oxalate monoanion, both of which are promoted by oxalate decarboxylase. Another intermedi ...[more]