Ontology highlight
ABSTRACT:
SUBMITTER: Gupta N
PROVIDER: S-EPMC4355579 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Gupta Neha N Culver Gloria M GM
Nature structural & molecular biology 20140907 10
Processing of transcribed precursor ribosomal RNA (pre-rRNA) to a mature state is a conserved aspect of ribosome biogenesis in vivo. We developed an affinity-purification system to isolate and analyze in vivo-formed pre-rRNA-containing ribonucleoprotein (RNP) particles (rRNPs) from wild-type E. coli. We observed that the first processing intermediate of pre-small subunit (pre-SSU) rRNA is a platform for biogenesis. These pre-SSU-containing RNPs have differing ribosomal-protein and auxiliary fact ...[more]