Ontology highlight
ABSTRACT:
SUBMITTER: Shimojo M
PROVIDER: S-EPMC7096426 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Communications biology 20200325 1
In vitro reconstitution is a powerful tool for investigating ribosome functions and biogenesis, as well as discovering new ribosomal features. In this study, we integrated all of the processes required for Escherichia coli small ribosomal subunit assembly. In our method, termed fully Recombinant-based integrated Synthesis, Assembly, and Translation (R-iSAT), assembly and evaluation of the small ribosomal subunits are coupled with ribosomal RNA (rRNA) synthesis in a reconstituted cell-free protei ...[more]