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Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1.

ABSTRACT: Myotubularin-related protein 1 is a phosphatase that dephosphorylates phospholipids such as phosphatidylinositol 3-phosphate or phosphatidylinositol 3,5-bisphosphate. In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277?K using polyethylene glycol 20,000 as a precipitant. Diffraction data were collected to 2.0?Å resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.581?Å, ? = 87.597, ? = 86.072, ? = 77.327°. Assuming the presence of four molecules in the asymmetric unit, the calculated Matthews coefficient value was 2.61?Å(3)?Da(-1) and the corresponding solvent content was 52.9%.

SUBMITTER: Bong SM

PROVIDER: S-EPMC4356299 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1.

Bong Seoung Min SM Yang Seung Won SW Choi Ji-Woong JW Kim Seung Jun SJ Lee Byung Il BI

Acta crystallographica. Section F, Structural biology communications 20150219 Pt 3

Myotubularin-related protein 1 is a phosphatase that dephosphorylates phospholipids such as phosphatidylinositol 3-phosphate or phosphatidylinositol 3,5-bisphosphate. In this study, human MTMR1 was overexpressed in Escherichia coli, purified and crystallized at 277 K using polyethylene glycol 20,000 as a precipitant. Diffraction data were collected to 2.0 Å resolution using synchrotron radiation. The crystals belonged to space group P1, with unit-cell parameters a = 67.219, b = 96.587, c = 97.58 ...[more]

PMID: 25760698

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