Ontology highlight
ABSTRACT:
SUBMITTER: Seelan RS
PROVIDER: S-EPMC4356551 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Seelan Ratnam S RS Warner Dennis R DR Mukhopadhyay Partha M PM Andres Sarah A SA Smolenkova Irina A IA Wittliff James L JL Michele Pisano M M Greene Robert M RM
Analytical biochemistry 20130730 1
Laser capture microdissection (LCM) is a superior method for nondestructive collection of specific cell populations from tissue sections. Although DNA, RNA, and protein have been analyzed from LCM-procured samples, epigenetic analyses, particularly of fetal, highly hydrated tissue, have not been attempted. A standardized protocol with quality assurance measures was established to procure cells by LCM of the medial edge epithelia (MEE) of the fetal palatal processes for isolation of intact microR ...[more]