G protein ?? subunits regulate cardiomyocyte hypertrophy through a perinuclear Golgi phosphatidylinositol 4-phosphate hydrolysis pathway.

ABSTRACT: We recently identified a novel GPCR-dependent pathway for regulation of cardiac hypertrophy that depends on Golgi phosphatidylinositol 4-phosphate (PI4P) hydrolysis by a specific isoform of phospholipase C (PLC), PLC?, at the nuclear envelope. How stimuli are transmitted from cell surface GPCRs to activation of perinuclear PLC? is not clear. Here we tested the role of G protein ?? subunits. G?? inhibition blocked ET-1-stimulated Golgi PI4P depletion in neonatal and adult ventricular myocytes. Blocking G?? at the Golgi inhibited ET-1-dependent PI4P depletion and nuclear PKD activation. Translocation of G?? to the Golgi stimulated perinuclear Golgi PI4P depletion and nuclear PKD activation. Finally, blocking G?? at the Golgi or PM blocked ET-1-dependent cardiomyocyte hypertrophy. These data indicate that G?? regulation of the perinuclear Golgi PI4P pathway and a separate pathway at the PM is required for ET-1-stimulated hypertrophy, and the efficacy of G?? inhibition in preventing heart failure maybe due in part to its blocking both these pathways.

SUBMITTER: Malik S 

PROVIDER: S-EPMC4357516 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature