Ontology highlight
ABSTRACT:
SUBMITTER: Blythe SA
PROVIDER: S-EPMC4359640 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Blythe Shelby A SA Wieschaus Eric F EF
Cell 20150305 6
A conserved feature of the midblastula transition (MBT) is a requirement for a functional DNA replication checkpoint to coordinate cell-cycle remodeling and zygotic genome activation (ZGA). We have investigated what triggers this checkpoint during Drosophila embryogenesis. We find that the magnitude of the checkpoint scales with the quantity of transcriptionally engaged DNA. Measuring RNA polymerase II (Pol II) binding at 20 min intervals over the course of ZGA reveals that the checkpoint coinci ...[more]