Ontology highlight
ABSTRACT:
SUBMITTER: Bhogaraju S
PROVIDER: S-EPMC4359902 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20130801 6149
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue exper ...[more]