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Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier.

ABSTRACT: Using and engineering amyloid as nanomaterials are blossoming trends in bionanotechnology. Here, we show our discovery of an amyloid structure, termed "amyloid-like nanosheet," formed by a key amyloid-forming segment of Alzheimer's A?. Combining multiple biophysical and computational approaches, we proposed a structural model for the nanosheet that is formed by stacking the amyloid fibril spines perpendicular to the fibril axis. We further used the nanosheet for laboratorial retroviral transduction enhancement and directly visualized the presence of virus on the nanosheet surface by electron microscopy. Furthermore, based on our structural model, we designed nanosheet-forming peptides with different functionalities, elucidating the potential of rational design for amyloid-based materials with novel architecture and function.

SUBMITTER: Dai B

PROVIDER: S-EPMC4364204 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Tunable assembly of amyloid-forming peptides into nanosheets as a retrovirus carrier.

Dai Bin B Li Dan D Xi Wenhui W Luo Fang F Zhang Xiang X Zou Man M Cao Mi M Hu Jun J Wang Wenyuan W Wei Guanghong G Zhang Yi Y Liu Cong C

Proceedings of the National Academy of Sciences of the United States of America 20150223 10

Using and engineering amyloid as nanomaterials are blossoming trends in bionanotechnology. Here, we show our discovery of an amyloid structure, termed "amyloid-like nanosheet," formed by a key amyloid-forming segment of Alzheimer's Aβ. Combining multiple biophysical and computational approaches, we proposed a structural model for the nanosheet that is formed by stacking the amyloid fibril spines perpendicular to the fibril axis. We further used the nanosheet for laboratorial retroviral transduct ...[more]

PMID: 25713359

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