Dataset Information

Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

ABSTRACT: Cardiac stem cells (CSCs) can differentiate into cardiac muscle?like cells; however, it remains unknown whether CSCs may possess the ability to differentiate into pacemaker cells. The aim of the present study was to determine whether angiotensin II (Ang II) could promote the specialization of CSCs into pacemaker?like cells. Mouse CSCs were treated with Ang II from day 3-5, after cell sorting. The differentiation potential of the cells was then analyzed by morphological analysis, flow cytometry, reverse transcription?polymerase chain reaction, immunohistochemistry and patch clamp analysis. Treatment with Ang II resulted in an increased number of cardiac muscle?like cells (32.7 ± 4.8% vs. 21.5 ± 4.8%; P<0.05), and inhibition of smooth muscle?like cells (6.2 ± 7.3% vs. 20.5 ± 5.1%; P<0.05). Following treatment with Ang II, increased levels of the cardiac progenitor?specific markers GATA4 and Nkx2.5 were observed in the cells. Furthermore, the transcript levels of pacemaker function?related genes, including hyperpolarization?activated cyclic nucleotide?gated (HCN)2, HCN4, T?box (Tbx)2 and Tbx3, were significantly upregulated. Immunofluorescence analysis confirmed the increased number of pacemaker?like cells. The pacemaker current (If) was recorded in the cells derived from CSCs, treated with Ang II. In conclusion, treatment of CSCs with Ang II during the differentiation process modified cardiac?specific gene expression and resulted in the enhanced formation of pacemaker?like cells.

SUBMITTER: Xue C

PROVIDER: S-EPMC4368082 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

Xue Cheng C Zhang Jun J Lv Zhan Z Liu Hui H Huang Congxin C Yang Jing J Wang Ten T

Molecular medicine reports 20150107 5

Cardiac stem cells (CSCs) can differentiate into cardiac muscle‑like cells; however, it remains unknown whether CSCs may possess the ability to differentiate into pacemaker cells. The aim of the present study was to determine whether angiotensin II (Ang II) could promote the specialization of CSCs into pacemaker‑like cells. Mouse CSCs were treated with Ang II from day 3-5, after cell sorting. The differentiation potential of the cells was then analyzed by morphological analysis, flow cytometry, ...[more]

PMID: 25572000

Similar Datasets

Project description:Long non-coding RNA (lncRNA) is receiving increasing attention in embryonic stem cells (ESCs) research. However, the roles of lncRNA in the differentiation of ESCs into pacemaker-like cells are still unclear. Therefore, the present study aims to explore the roles and mechanisms of lncRNA in the differentiation of ESCs into pacemaker-like cells. ESCs were cultured and induced differentiation to pacemaker-like cells. RNA sequencing was used to identify the differential expression lncRNAs during the differentiation of ESCs into pacemaker-like cells. Cell morphology observation, flow cytometry, quantitative real-time polymerase chain reaction, western blot, and immunofluorescence were used to detect the differentiation of ESCs into pacemaker-like cells. LncRNA and genes overexpression or knockdown through transfected adenovirus in the differentiation process. The fluorescence in situ hybridization (FISH) detected the lncRNA location in the differentiated ESCs. Luciferase reporter gene assay, methylation-specific PCR, chromatin immunoprecipitation assay, and RNA immunoprecipitation assay were performed to reveal the mechanism of lncRNA-regulating HCN4 expression. Rescue experiments were used to confirm that lncRNA regulates the differentiation of ESCs into pacemaker-like cells through HCN4. We cultured the ESCs and induced the differentiation of ESCs into pacemaker-like cells successfully. The expression of lncRNA RCPCD was significantly decreased in the differentiation of ESCs into pacemaker-like cells. Overexpression of RCPCD inhibited the differentiation of ESCs into pacemaker-like cells. RCPCD inhibited the expression of HCN4 by increasing HCN4 methylation at the promoter region through DNMT1, DNMT2, and DNMT3. RCPCD inhibited the differentiation of ESCs into pacemaker-like cells by inhibiting the expression of HCN4. Our results confirm the roles and mechanism of lncRNA RCPCD in the differentiation of ESCs into pacemaker-like cells, which could pave the path for the development of a cell-based biological pacemaker.

Dataset Information

Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

Publications

Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets