Unknown

Dataset Information

0

Metabolic engineering of medium-chain fatty acid biosynthesis in Nicotiana benthamiana plant leaf lipids.


ABSTRACT: Various research groups are investigating the production of oil in non-seed biomass such as leaves. Recently, high levels of oil accumulation have been achieved in plant biomass using a combination of biotechnological approaches which also resulted in significant changes to the fatty acid composition of the leaf oil. In this study, we were interested to determine whether medium-chain fatty acids (MCFA) could be accumulated in leaf oil. MCFA are an ideal feedstock for biodiesel and a range of oleochemical products including lubricants, coatings, and detergents. In this study, we explore the synthesis, accumulation, and glycerolipid head-group distribution of MCFA in leaves of Nicotiana benthamiana after transient transgenic expression of C12:0-, C14:0-, and C16:0-ACP thioesterase genes. We demonstrate that the production of these MCFA in leaf is increased by the co-expression of the WRINKLED1 (WRI1) transcription factor, with the lysophosphatidic acid acyltransferase (LPAAT) from Cocos nucifera being required for the assembly of tri-MCFA TAG species. We also demonstrate that the newly-produced MCFA are incorporated into the triacylglycerol of leaves in which WRI1 + diacylglycerol acyltransferase1 (DGAT1) genes are co-expressed for increased oil accumulation.

SUBMITTER: Reynolds KB 

PROVIDER: S-EPMC4371700 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Metabolic engineering of medium-chain fatty acid biosynthesis in Nicotiana benthamiana plant leaf lipids.

Reynolds Kyle B KB   Taylor Matthew C MC   Zhou Xue-Rong XR   Vanhercke Thomas T   Wood Craig C CC   Blanchard Christopher L CL   Singh Surinder P SP   Petrie James R JR  

Frontiers in plant science 20150324


Various research groups are investigating the production of oil in non-seed biomass such as leaves. Recently, high levels of oil accumulation have been achieved in plant biomass using a combination of biotechnological approaches which also resulted in significant changes to the fatty acid composition of the leaf oil. In this study, we were interested to determine whether medium-chain fatty acids (MCFA) could be accumulated in leaf oil. MCFA are an ideal feedstock for biodiesel and a range of ole  ...[more]

Similar Datasets

| S-EPMC8804313 | biostudies-literature
| S-EPMC4208727 | biostudies-literature
| S-EPMC6813417 | biostudies-literature
| S-EPMC6130074 | biostudies-literature
| S-EPMC6230952 | biostudies-literature
| S-EPMC4446107 | biostudies-literature
| S-EPMC8564105 | biostudies-literature
| S-EPMC4032284 | biostudies-literature
| S-EPMC5808347 | biostudies-literature
| S-EPMC7502760 | biostudies-literature