Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC6813417 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Li Jianhua J Mutanda Ishmael I Wang Kaibo K Yang Lei L Wang Jiawei J Wang Yong Y
Nature communications 20191024 1
Production of the anticancer drug Taxol and its precursors in heterologous hosts is more sustainable than extraction from tissues of yew trees or chemical synthesis. Although attempts to engineer the Taxol pathway in microbes have made significant progress, challenges such as functional expression of plant P450 enzymes remain to be addressed. Here, we introduce taxadiene synthase, taxadiene-5α-hydroxylase, and cytochrome P450 reductase in a high biomass plant Nicotiana benthamiana. Using a chlor ...[more]