Ontology highlight
ABSTRACT:
SUBMITTER: Rivera-Fuentes P
PROVIDER: S-EPMC4372157 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Rivera-Fuentes Pablo P Wrobel Alexandra T AT Zastrow Melissa L ML Khan Mustafa M Georgiou John J Luyben Thomas T TT Roder John C JC Okamoto Kenichi K Lippard Stephen J SJ
Chemical science 20150101 3
Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn<sup>2+</sup> in lysosomes of HeLa cells, endogenous Zn<sup>2+</sup> in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wa ...[more]