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A Far-Red Emitting Probe for Unambiguous Detection of Mobile Zinc in Acidic Vesicles and Deep Tissue.

ABSTRACT: Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn²⁺ in lysosomes of HeLa cells, endogenous Zn²⁺ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 µm, as demonstrated by studies in live tissue employing two-photon microscopy.

SUBMITTER: Rivera-Fuentes P

PROVIDER: S-EPMC4372157 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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A Far-Red Emitting Probe for Unambiguous Detection of Mobile Zinc in Acidic Vesicles and Deep Tissue.

Rivera-Fuentes Pablo P Wrobel Alexandra T AT Zastrow Melissa L ML Khan Mustafa M Georgiou John J Luyben Thomas T TT Roder John C JC Okamoto Kenichi K Lippard Stephen J SJ

Chemical science 20150101 3

Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn<sup>2+</sup> in lysosomes of HeLa cells, endogenous Zn<sup>2+</sup> in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wa ...[more]

PMID: 25815162

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