Ontology highlight
ABSTRACT:
SUBMITTER: Bloomfield G
PROVIDER: S-EPMC4374526 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Bloomfield Gareth G Traynor David D Sander Sophia P SP Veltman Douwe M DM Pachebat Justin A JA Kay Robert R RR
eLife 20150327
Cells use phagocytosis and macropinocytosis to internalise bulk material, which in phagotrophic organisms supplies the nutrients necessary for growth. Wildtype Dictyostelium amoebae feed on bacteria, but for decades laboratory work has relied on axenic mutants that can also grow on liquid media. We used forward genetics to identify the causative gene underlying this phenotype. This gene encodes the RasGAP Neurofibromin (NF1). Loss of NF1 enables axenic growth by increasing fluid uptake. Mutants ...[more]