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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

ABSTRACT: The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

SUBMITTER: Collot M

PROVIDER: S-EPMC4379494 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

Collot Mayeul M Wilms Christian D CD Bentkhayet Asma A Marcaggi Païkan P Couchman Kiri K Charpak Serge S Dieudonné Stéphane S Häusser Michael M Feltz Anne A Mallet Jean-Maurice JM

eLife 20150331

The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imag ...[more]

PMID: 25824291

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Dataset Information

CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

Publications

CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

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