Reverse gyrase is not a prerequisite for hyperthermophilic life.
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ABSTRACT:
SUBMITTER: Atomi H
PROVIDER: S-EPMC438624 | biostudies-literature | 2004 Jul
REPOSITORIES: biostudies-literature
Publications
Reverse gyrase is not a prerequisite for hyperthermophilic life.
Atomi Haruyuki H Matsumi Rie R Imanaka Tadayuki T
Journal of bacteriology 20040701 14
We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90 degrees C. This study provides experimental evidence that reverse ...[more]
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