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Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013.

ABSTRACT: We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lomé in Togo. Influenza A virus genome was detected by RT-PCR in 2.5-12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eight genes similar to A(H1N1)pdm09 virus genes circulating in humans in 2012-2013, suggesting human-to-swine transmission of the pathogen.

SUBMITTER: Ducatez MF

PROVIDER: S-EPMC4388795 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Influenza A(H1N1)pdm09 virus in pigs, Togo, 2013.

Ducatez Mariette F MF Awoume Félix F Webby Richard J RJ

Veterinary microbiology 20150304 1-2

We collected 325 nasal swabs from freshly slaughtered previously healthy pigs from October 2012 through January 2014 in a slaughterhouse near Lomé in Togo. Influenza A virus genome was detected by RT-PCR in 2.5-12.3% of the pooled samples, and results of hemagglutinin subtyping RT-PCR assays showed the virus in all the positive pools to be A(H1N1)pdm09. Virus was isolated on MDCK cells from a representative specimen, A/swine/Togo/ONA32/2013(H1N1). The isolate was fully sequenced and harbored eig ...[more]

PMID: 25778544

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