Ontology highlight
ABSTRACT:
SUBMITTER: Otten C
PROVIDER: S-EPMC4388811 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Otten Christian C De Benedetti Stefania S Gaballah Ahmed A Bühl Henrike H Klöckner Anna A Brauner Jarryd J Sahl Hans-Georg HG Henrichfreise Beate B
PloS one 20150407 4
Heterologous overexpression of foreign proteins in Escherichia coli often leads to insoluble aggregates of misfolded inactive proteins, so-called inclusion bodies. To solve this problem use of chaperones or in vitro refolding procedures are the means of choice. These methods are time consuming and cost intensive, due to additional purification steps to get rid of the chaperons or the process of refolding itself. We describe an easy to use lab-scale method to avoid formation of inclusion bodies. ...[more]