Ontology highlight
ABSTRACT:
SUBMITTER: Prasad RM
PROVIDER: S-EPMC4392510 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Prasad Rahul M RM Jin Xingjian X Aboualaiwi Wissam A WA Nauli Surya M SM
Biological procedures online 20140331 1
<h4>Background</h4>Cell-based perfusion studies have provided great insight into fluid-sensing mechanisms, such as primary cilia in the renal and vascular systems. However, the intrinsic limitations of in vitro cell culture, such as the inability to reflect cellular organization within tissues, has distanced observed paradigms from possible clinical developments. Here we describe a protocol that applies ex vivo artery perfusion and calcium imaging to observe real-time cellular responses to fluid ...[more]