Homogeneous low-molecular-weight heparins with reversible anticoagulant activity.
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ABSTRACT:
SUBMITTER: Xu Y
PROVIDER: S-EPMC4393012 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
Homogeneous low-molecular-weight heparins with reversible anticoagulant activity.
Xu Yongmei Y Cai Chao C Chandarajoti Kasemsiri K Hsieh Po-Hung PH Li Lingyun L Pham Truong Q TQ Sparkenbaugh Erica M EM Sheng Juzheng J Key Nigel S NS Pawlinski Rafal R Harris Edward N EN Linhardt Robert J RJ Liu Jian J
Nature chemical biology 20140223 4
Low-molecular-weight heparins (LMWHs) are carbohydrate-based anticoagulants clinically used to treat thrombotic disorders, but impurities, structural heterogeneity or functional irreversibility can limit treatment options. We report a series of synthetic LMWHs prepared by cost-effective chemoenzymatic methods. The high activity of one defined synthetic LMWH against human factor Xa (FXa) was reversible in vitro and in vivo using protamine, demonstrating that synthetically accessible constructs ca ...[more]
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