Ontology highlight
ABSTRACT:
SUBMITTER: Ran FA
PROVIDER: S-EPMC4393360 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Ran F Ann FA Cong Le L Yan Winston X WX Scott David A DA Gootenberg Jonathan S JS Kriz Andrea J AJ Zetsche Bernd B Shalem Ophir O Wu Xuebing X Makarova Kira S KS Koonin Eugene V EV Sharp Phillip A PA Zhang Feng F
Nature 20150401 7546
The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly versatile adeno-associated virus (AAV) delivery vehicle. Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more t ...[more]