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An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis.


ABSTRACT: Mycobacterium tuberculosis encodes a proteasome that is highly similar to eukaryotic proteasomes and is required to cause lethal infections in animals. The only pathway known to target proteins for proteasomal degradation in bacteria is pupylation, which is functionally analogous to eukaryotic ubiquitylation. However, evidence suggests that the M. tuberculosis proteasome contributes to pupylation-independent pathways as well. To identify new proteasome cofactors that might contribute to such pathways, we isolated proteins that bound to proteasomes overproduced in M. tuberculosis and found a previously uncharacterized protein, Rv3780, which formed rings and capped M. tuberculosis proteasome core particles. Rv3780 enhanced peptide and protein degradation by proteasomes in an adenosine triphosphate (ATP)-independent manner. We identified putative Rv3780-dependent proteasome substrates and found that Rv3780 promoted robust degradation of the heat shock protein repressor, HspR. Importantly, an M. tuberculosis Rv3780 mutant had a general growth defect, was sensitive to heat stress, and was attenuated for growth in mice. Collectively, these data demonstrate that ATP-independent proteasome activators are not confined to eukaryotes and can contribute to the virulence of one the world's most devastating pathogens.

SUBMITTER: Jastrab JB 

PROVIDER: S-EPMC4394314 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis.

Jastrab Jordan B JB   Wang Tong T   Murphy J Patrick JP   Bai Lin L   Hu Kuan K   Merkx Remco R   Huang Jessica J   Chatterjee Champak C   Ovaa Huib H   Gygi Steven P SP   Li Huilin H   Darwin K Heran KH  

Proceedings of the National Academy of Sciences of the United States of America 20150323 14


Mycobacterium tuberculosis encodes a proteasome that is highly similar to eukaryotic proteasomes and is required to cause lethal infections in animals. The only pathway known to target proteins for proteasomal degradation in bacteria is pupylation, which is functionally analogous to eukaryotic ubiquitylation. However, evidence suggests that the M. tuberculosis proteasome contributes to pupylation-independent pathways as well. To identify new proteasome cofactors that might contribute to such pat  ...[more]

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